Premium
In vitro Evaluation of Wild Fruits and Leaves for Potential Carbohydrate Hydrolyzing Enzyme Inhibition
Author(s) -
Lee Diana,
Racicot Kenneth,
Apostolidis Emmanouil
Publication year - 2015
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.29.1_supplement.924.26
Subject(s) - dandelion , dpph , chemistry , antioxidant , taraxacum officinale , food science , carbohydrate , alpha glucosidase , amylase , enzyme , traditional medicine , botany , biochemistry , biology , medicine , alternative medicine , pathology , traditional chinese medicine
Four wild foods dandelion leaf ‐ ( Taraxacam officinale ), grape ( Vitis riparia ) plantain ( Plantago major ), and beach plum ( Prunus maritima ) ‐, were extracted in water followed by C 18 column extraction. The phenolic content was evaluated using the Folin‐Ciocalteau's Assay and determined to be 39.5 μg/mg GAEq, 116.2 μg/mg GAEq, 14.3 μg/mg GAEq, and 351.1 μg/mg GAEq for the dandelion, grape, plantain, and plum respectively. The antioxidant activity was evaluated in terms of free‐radical scavenging activity, by using the DPPH assay. Single dose evaluation showed that the sample with the highest free‐radical scavenging activity was the beach plum with 70.2% antioxidant activity, followed by grape with (58.6%), dandelion (50.0%), and plantain (44.9%). Dose‐dependent evaluation of free‐radical scavenging activity is currently underway. Previous reports have shown that phenolic phytochemicals are excellent inhibitors of carbohydrate hydrolyzing enzymes. To determine the possible effect of our samples for type 2 diabetes prevention, we will evaluate their dose‐dependent inhibitory effect against two major carbohydrate hydrolyzing enzymes (alpha‐glucosidase and alpha‐amylase). Completion of this research will provide the basic biochemical rationale for the deeper evaluation of samples with high inhibitory activity.