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Effect of oral administration of DHA on the remodeling of calcium handling proteins in congestive heart failure in male rats
Author(s) -
Yamanushi Tomoko,
Kabuto Hideaki,
Akiyama Kaoru,
Tsushima Tadahiro,
Misawa Yoshihisa
Publication year - 2015
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.29.1_supplement.923.12
Subject(s) - serca , heart failure , ryanodine receptor , medicine , endocrinology , calcium , endoplasmic reticulum , saline , chemistry , atpase , calcium metabolism , cardiac function curve , calcium in biology , enzyme , biochemistry
Calcium (Ca 2+ ) cycling is essential in cardiac contractile function, and remodeling of calcium handling is thought to be one of the factors contributing to the dysfunction in heart failure (HF). This study investigated the effect of DHA administration on remodeling of Ca 2+ handling proteins in congestive heart failure (CHF). Male rats were divided into 4 groups (n=8 for each group): C (normal, administered saline); D (normal, administered DHA‐ethyl ester (DHA‐Et)); H (CHF, administered saline); HD (CHF, administered DHA‐Et). CHF was produced by injection of monocrotaline (MCT) into 5‐week‐old rats. Starting from 3 days before MCT injection, saline or DHA‐Et wasorally administered(1g/kg body weight) daily. The right ventriclewas removed on day 24, and quantitative PCR was used to measure the expression of transcripts.The expression of sarcoplasmic reticulum Ca 2+ ‐ATPase (SERCA) 2a, which is responsible to transport back Ca 2+ to sarcoplasmic reticulum (SR), was significantly decreased in group H compared to groups C, D and HD (P 蠄 0.05). The expression of ryanodine receptor (RYR) 3, which releases Ca 2+ from SR, in group H was significantly increased compared to groups C and D (P 蠄 0.05), and also tended to be increased in group H compared to group HD (P=0.06).The expression of Ca v 1.2, a component of L‐type Ca 2+ channel,was significantly decreased in group H compared to groups C and D (P 蠄 0.05). Remodeling of intracellular Ca 2+ handling may be caused by these changes in gene expression in CHF. Administration of DHA‐Et may improve abnormal Ca 2+ handling through recovery of the expression of these proteins.