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Dehydroglyasperin C Suppresses NF‐κB‐dependent Inflammation from Acute Lung Injury through Nrf2 Activation in mice
Author(s) -
Seo Ji Yeon,
Ji Hyun Kyung,
Kim Min Sun,
Kang Su Jin,
Jo Gwang Rae,
Erdenebileg Zolzaya,
Lim Soon Sung,
Kim JongSang
Publication year - 2015
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.29.1_supplement.922.21
Subject(s) - glycyrrhiza uralensis , inflammation , macrophage , western blot , secretion , tumor necrosis factor alpha , pharmacology , lipopolysaccharide , proinflammatory cytokine , nf κb , immunology , medicine , chemistry , biochemistry , pathology , in vitro , alternative medicine , gene
Glycyrrhiza uralensis (or licorice) is a widely used Oriental herbal medicine as well as food additive. Licorice has been reported to have therapeutic effects on malaria, cardiovascular disease, peptic ulcers, hepatitis C, and pulmonary and skin diseases. In this study, we intended to figure out anti‐inflammatory activities of prenyflavonoids in RAW264.7 macrophage and C57BL/6 mice. In order to induce inflammation, we treated lipopolysaccharide (LPS) to RAW264.7 macrophage, C57BL/6 wild type and Nrf2 knockout mice. The secretion of TNFα, an inflammatory cytokine, was determined by using collected cell culture medium and mouse plasma. Phosphorylated nuclear factor (NF)‐kappa B and cyclo‐oxygenase‐2 (COX‐2) were assessed by Western blot analysis. The translocation of pNFκB into nucleus was determined by immunocytochemistry in RAW264.7 cells. The results indicated that DGC and IsoA translocated phosphorylated NFκB from nucleus to cytoplasm and prenylflavonoids decreased COX‐2 expression. In addition, DGC lowered the LPS‐increased TNFα secretion in plasma. In conclusion, prenylflavonoids merit further study to evaluate their practical usefulness as anti‐inflammatory agents.