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Functional Studies of the Putative Organic Cation Transporter SLC22A23
Author(s) -
Chaity Nazia
Publication year - 2015
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.29.1_supplement.915.2
Subject(s) - solute carrier family , green fluorescent protein , gene , transporter , biology , transfection , subcellular localization , gene expression , inflammatory bowel disease , membrane protein , hek 293 cells , transport protein , microbiology and biotechnology , genetics , disease , medicine , pathology , membrane
SLC22A23 (Solute Carrier 22 family 23) is an orphan gene that encodes for a putative organic cation membrane transporter protein. The gene has been repeatedly associated to inflammatory bowel disease however; the gene structure in human and its function is still uncharacterized. Inflammatory bowel disease (IBD) is a chronic disease that is steadily increasing worldwide with the highest prevalence in Canada. Objectives The proposed research aims to determine the tissue expression and characterize the subcellular location of the SLC22A23 protein, in mammalian cells. Methods Human SLC22A23 has been subcloned by Gateway® Technology and tagged with green fluorescent protein (GFP) and transfected in three cell lines‐ CHO, MDCK and Caco‐2. Membrane localization of SLC22A23 has been performed using confocal fluorescence microscopy. And to determine the tissue expression Real‐Time polymerase chain reaction has been used. Results Human SLC22A23 protein is located in the cell membrane and is abundantly expressed in various tissues in human body with high expression in brain, kidney and intestine. Conclusion The characterization of the SLC22A23 gene will provide the basis to determine which genetic variation is causative in the development of IBD in future studies. The correct sub‐cellular location will confirm that the human SLC22A23 is a membrane transporter protein (as known from the rat orthologous). Further functional studies of this gene will explicate the mechanism in the development of IBD and will contribute to the understanding which metabolite/nutrient imbalance plays a role in the development of IBD.