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Ferulic Acid Ethyl Ester Attenuates Inflammatory Response by Suppressing ROS‐related NF‐κB Signaling Pathway in LPS‐induced RAW264.7 Cells
Author(s) -
Seo MinJi,
Kim JinKyoung,
Lee SangHyun,
Wang FangFang,
Kang YuRi,
Kang MyungHee,
Kwon YoungIn,
Jang HaeDong
Publication year - 2015
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.29.1_supplement.913.15
Subject(s) - chemistry , ferulic acid , mapk/erk pathway , signal transduction , kinase , nf κb , p38 mitogen activated protein kinases , lipid signaling , lipopolysaccharide , proinflammatory cytokine , pharmacology , phosphorylation , biochemistry , microbiology and biotechnology , inflammation , receptor , immunology , biology
Ferulic acid ethyl ester (FAEE) is an ester derivative of ferulic acid and the latter is known for its anti‐inflammatory and antioxidant properties. Previous studies have shown that ferulic acid protects synaptosomal membrane system and neuronal cell culture systems against hydroxyl and peroxyl radical oxidation. FAEE is lipophilic and is able to easily penetrate into lipid bilayer. Therefore, the present study was designed to investigate the anti‐inflammatory effects of FAEE and its underlying mechanism in RAW264.7 cells. FAEE attenuated the release of NO, PGE 2 and pro‐inflammatory cytokines such as IL‐1β, IL‐6 and TNF‐α in a dose‐dependent manner. Consistent with NO and PGE 2 inhibition, FAEE suppressed LPS‐induced iNOS and COX‐2 expression as well as the promoter activities of COX‐2 and iNOS. In addition, FAEE pre‐treatment ameliorated ROS and superoxide anion levels in a time‐dependent manner. FAEE also dose‐dependently diminished LPS‐induced the activation mitogen‐activated protein kinases (MAPK) including JNK, ERK and p‐38 by phosphorylation. Furthermore, FAEE pre‐treatment suppressed the activation and translocation into the nucleus of NF‐κB in a dose‐dependent manner by western blotting and confocal microscopic abservation. The overall results indicate that FAEE may attenuate the inflammatory response of LPS‐induced RAW264.7 cells through ROS‐related MAPK/NF‐κB signaling pathway.