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Immunomodulatory effects of methyl 2‐[propanamide‐2'‐methoxycarbonyl]‐benzoate (MPMB) in macrophages: involvement of Akt/PI3k/NF‐κB signaling pathway
Author(s) -
Lee Hee Weon,
Kwon Il Seoul
Publication year - 2015
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.29.1_supplement.913.11
Subject(s) - helianthus , pi3k/akt/mtor pathway , western blot , protein kinase b , chemistry , nf κb , macrophage , tumor necrosis factor alpha , mtt assay , biochemistry , signal transduction , microbiology and biotechnology , biology , cell growth , immunology , in vitro , sunflower , gene , horticulture
Helianthus tuberosus. L is also known as Jerusalem artichoke, sunroot or earth apple. The tubers of Helianthus tuberosus. L are already used as root vegetable and dietary foods. However, the effect of MPMB on macrophage function remains unknown. A compound whose chemical name is methyl 2‐[propanamide‐2'‐methoxycarbonyl]‐benzoate (MPMB)has been extracted from the tuber of Helianthus tuberosus. L . In the present study, we observed an immunomodulating effect of MPMB in macrophages. MTT assay revealed that MPMBis not cytotoxic at 100 μg/ml of MPMB. The production of pro‐inflammatory cytokines, TNF‐ααand IL‐6, was significantly reduced in a dose‐dependent manner which is confirmed by ELISA. In addition, western blot analysis showed that pre‐treatment of MPMB for 2h suppressed the expression of Akt, PI3k and CK2α. Furthermore, immunoblot analysis demonstrated that MPMB reduced nuclear translocation of p65 by inhibiting degradation of Iκb‐α. Taken together, these results suggest that MPMB has an immunomodulating effect on macrophage secretory and cellular activities and is a novel compound to treat inflammatory diseases.