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Calcium‐dependent Chemotherapeutic Mechanisms of Resveratrol
Author(s) -
Kenealey Jason
Publication year - 2015
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.29.1_supplement.897.26
Subject(s) - resveratrol , calcium , cytotoxicity , apoptosis , chemistry , cytosol , cell , cancer cell , microbiology and biotechnology , calcium metabolism , cytoplasm , cancer research , cancer , biochemistry , biology , in vitro , organic chemistry , genetics , enzyme
In 2013 breast cancer killed approximately 40,000 Americans due in part to the ineffectiveness of chemotherapy. Current chemotherapeutic options are limited by their cytotoxicity to normal tissue and variable efficacy. Resveratrol is a non‐toxic natural product that is selectively deleterious to tumor cells. However, what is not understood is how resveratrol targets tumor cells. We use live cell microscopy to show that an apoptotic rise in cytoplasmic calcium concentration ([Ca 2+ ] I ) is induced by resveratrol in cancer cells, but not in normal breast cells. Further, we demonstrate that the change in fura‐2 fluorescence is due to a change in [Ca 2+ ] I and not an increase due to overlapping fluorescent spectra. Further, we have evidence that the mobilization of ([Ca 2+ ] I ) by resveratrol activates additional apoptotic mechanisms. These data demonstrate that resveratrol stimulates a tumor cell specific apoptotic flux of calcium into the cytosol that stimulates apoptotic signaling