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Doxorubicin‐coupled MNPs‐Fe 3 O 4 with Electromagnetic Radiation Improve the Effectiveness of Doxorubicin on the T47D Human Ductal Breast Tumor Cell Line
Author(s) -
Alobaid Sarah,
Sinha Saion,
Rossi Michael
Publication year - 2015
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.29.1_supplement.897.13
Subject(s) - doxorubicin , cytotoxicity , chemistry , cancer research , magnetic nanoparticles , breast cancer , drug delivery , iron oxide nanoparticles , pharmacology , chemotherapy , in vitro , cancer cell , cancer , biophysics , nanoparticle , medicine , nanotechnology , materials science , biochemistry , biology , organic chemistry
Chemotherapeutic and anticancer drugs are limited by their serious side effects. Doxorubicin (DOX) is an anticancer compound that is used to treat breast cancer, but it can lead to irreversible and harmful side effects. Therefore, finding more effective and specific delivery methods may enhance the efficacy of DOX and decrease its side effects. Magnetic iron oxide (Fe 3 O 4 ) particles (160 nm) were coupled with DOX and magnetically delivered to T47D breast cancer cells. The aim of this study is to develop a drug delivery system and to examine the effects of using magnetic nanoparticles (MNPs) on T47D breast cancer cells in vitro . T47D cells were treated with DOX, magnetic nanoparticles (MNPs), and with the DOX‐coupled magnetic nanoparticles complexes (DOX‐MNP) in present and absent of magnetic fields. Results showed that the cytotoxicity of DOX alone against T47D breast cancer cells was similar to the cytotoxic of DOX‐MNP after two days of treatment. However, assessing the cytotoxicity over longer time periods found that DOX‐MNP nanoparticles resulted in a greater decrease in cell viability compared to DOX treatment alone. The largest difference was seen after the five and six days of treatment. Cellular uptake of the complexes DOX‐MNP was increased by increasing the exposure time to the magnetic field. This was mirrored by an increase in the DOX‐MNP induced cytotoxicity. These results suggest that the use of chemotherapeutic compounds coupled to MNP may be an effective mechanism for their delivery to tumor cells allowing greater cytotoxicity at potentially lower doses.