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Role of calcium in regulating the production of urine by the Malpighian tubules of Rhodnius prolixus.
Author(s) -
Hassan Noman,
Gioino Paula,
Katselis George,
Ianowski Juan
Publication year - 2015
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.29.1_supplement.896.8
Subject(s) - intracellular , ion transporter , microbiology and biotechnology , chemistry , ion channel , calcium in biology , malpighian tubule system , rhodnius prolixus , serca , tubule , endocrinology , biology , receptor , biochemistry , kidney , ecology , botany , membrane , larva , insect , midgut , atpase , enzyme
Background R. prolixus Malpighian tubules require very efficient transport machinery and regulatory mechanisms to prevent catastrophic imbalances in intracellular ion composition. Recently, we have demonstrated that tubules stimulated with the diuretic hormone serotonin (5HT) trigger intracellular calcium (Ca 2+ ) waves that play a pivotal role in the regulation of ion transport .Questions: What is the intracellular second messenger pathway triggered by 5HT? What is coded in the intracellular Ca 2+ waves? Methods Mass spectrometry (MS) techniques were employed to analyze the changes in intracellular proteome between non‐stimulated (control) and 5HT‐stimulated tubules. The effect of individual protein changes on the regulation in M. tubules was studied using pharmcology . Intracellular Ca 2+ imaging was used to study the effect of altering the ion transport properties of the tubule on 5HT‐stimulated Ca 2+ waves. Results 5HT‐stimulated intracellular Ca 2+ signaling are blocked by treatment with PLC and IP3 receptor inhibitors. Ca 2+ waves are modulated by blockers of ion transporters (bumetanide). MS analysis showed differences between control and 5HT‐stimulated groups. We detected kinases WNK and OSR1 that have been shown to regulate ion transport in vertebrate system. This is the first time that these kinases are reported in R. Prolixus . Conclusion Intracellular Ca 2+ is triggered by PLC and IP3 and modulated by ion and fluid flux. We propose that the amplitude modulation of the Ca 2+ signal may be important in coding for the ion transport state of the cells. The global proteomics analysis indicates that WNK and OSR1 may be involved in the regulation of 5HT‐stimulated fluid secretion.