Premium
Systems Analysis of Soybean Seed Development; Lipid Body Ontogeny
Author(s) -
Miernyk Jan,
Wilson Rashaun,
Johnston Mark,
Thelen Jay
Publication year - 2015
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.29.1_supplement.887.9
Subject(s) - proteome , endoplasmic reticulum , proteomics , organelle , germination , biology , seedling , ontogeny , botany , biochemistry , microbiology and biotechnology , chemistry , gene , genetics
During seed development plants typically store carbon in the form of triacylglycerides within specialized subcellular organelles called oil or lipid bodies. This oil is mobilized and used for anaplerotic reactions that are necessary between seed germination and the young seedling becoming autotrophic. Lipid bodies (LB) are formed by vesiculation from the endoplasmic reticulum, and uniquely have a half‐unit membrane. This study was undertaken in order to determine if there are changes in the oil body proteome during seed development. We have separated soybean seed development into nine well‐defined stages. Oil accumulation begins at S4. The LB were isolated and purified from stages S4 through S9. Proteins were extracted from the purified LB, and analyzed by nLC plus tandem mass spectrometry. We were able to identify a cohort of proteins common to all developmental stages, and performed semi‐quantitative analysis of these proteins to determine relative changes in abundance. Proteins unique to each stage were also identified. The proteomics results will be combined with results from transcript profiling to provide a platform for systems analysis of soybean seed development.