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Target and Non‐target metabolomics profiling of different barley varieties affected by enhanced ultraviolet radiation and various C:N stoichiometry
Author(s) -
Oravec Michal,
Novotná Kateřina,
Rajsnerová Petra,
Veselá Barbora,
Urban Otmar,
Holub Petr,
Klem Karel
Publication year - 2015
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.29.1_supplement.887.7
Subject(s) - metabolomics , chemistry , metabolome , apigenin , polyphenol , abiotic component , food science , luteolin , biochemistry , biophysics , flavonoid , biology , antioxidant , chromatography , paleontology
Metabolomic profiling represents a new tool to ascertain the plants response to biotic or abiotic stresses. Generally, biosynthesis and accumulation of metabolites especially polyphenols in plants is induced by enhanced UV radiation and nitrogen deficiency. However, the spectrum of polyphenols, their localizations, and dynamics of synthesis/degradation may be altered by UV:PAR ratio and C:N stoichiometry. The main objective of our work was to study how changes in UV:PAR ratio (modulated by UVA+UVB lamps and plastic filters) and N supply influence metabolic profiles in barley varieties with different sensitivity to oxidative stress. Metabolomic profiling (HPLC‐MS), accompanied by physiological measurements, covered a wide spectrum of secondary and primary metabolites. Significant differences in constitutive metabolomic profiles, particularly phenolics, were observed between barley varieties. High UVA and UVB doses increased synthesisof galangin, luteolin, chrysin etc., particularly in sensitive Barke variety. On the contrary, high N supply reduced concentrationof sinapic acid, homoorientin, apigenin etc., particularly in resistant Bonus variety. Such decrease in phenolics related to reduction of photosynthetic parameters induced by UV radiation. Acknowledgement This work is part of research supported by grant M200871201 (Academy of Sciences, CR)