Premium
Discovery of Ceramide 1‐Phosphate Binding Proteins
Author(s) -
Shirey Carolyn,
Stahelin Robert
Publication year - 2015
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.29.1_supplement.886.7
Subject(s) - ceramide , microbiology and biotechnology , sphingolipid , lipid signaling , biology , chemistry , biochemistry , receptor , apoptosis
Intro Sphingolipids are a class of biomolecules that play key roles in cellular signaling and membrane trafficking, with main players including sphingosine, sphingosine‐1‐phosphate (S1P), ceramide, and ceramide‐1‐phosphate (C1P). C1P is an important metabolite that has been shown to regulate cell proliferation, phagocytosis, and macrophage chemotaxis. It also serves as a pro‐inflammatory signal and has been implicated in tumor metastasis and myocardial infarction. However, little is known about the specific role of C1P and its binding mechanisms in these processes, and the need for further study is evident. Study Objective My project aims to explore the proteins that specifically bind to C1P. We have also been exploring the properties of a fluorescently labeled C1P, to determine if the labeled C1P functions the same as natural C1P in cells. Methods Immunoprecipitation assays with lipid‐coated beads, mass spectrometry, confocal microscopy, and fluorescence assays. Results The fluorescently labeled C1P binds and activates proteins in a similar fashion to C1P in cells. This is a tool we will be able to use in the future for investigating binding and activation of proteins, and membrane dynamics of C1P. Conclusions C1P is gaining importance as a bioactive sphingolipid, as it plays many important roles within the cell. We hope to compile a comprehensive list of C1P specific binding proteins in order to elucidate many of these cell processes. Financial Support NSF‐GRFP Grant No.DGE‐1313583