Premium
Ablation of Perilipin 1 Alters Whole Body Glucose Homeostasis
Author(s) -
Sohn Jee Hyung,
Lee Yun Kyung,
Han Ji Seul,
Kim Jae Bum
Publication year - 2015
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.29.1_supplement.885.15
Subject(s) - perilipin , lipolysis , lipid droplet , medicine , endocrinology , glucose homeostasis , lipid metabolism , insulin resistance , hormone sensitive lipase , biology , chemistry , insulin , adipose tissue
Lipid droplets are cellular organelles that enclose neutral lipid depots and play dynamic roles in lipid metabolism by lipid droplet proteins. Perilipin 1 is one of the major lipid droplet binding proteins in adipocytes. Perilipin1 protein is phosphorylated by protein kinase A and its phosphorylation is necessary for translocation of hormone‐sensitive lipase to the lipid droplets to facilitate lipolysis. As well known, depletion of perilipin 1 gene in mice is resistance to diet‐induced obesity as well as increases basal lipolysis and reduces stimulated lipolysis. However, it is largely unknown whether insulin signaling is changed in perilipin 1 knockout (Plin1 KO) mice compared with wild type (WT) mice. To investigate potential roles for perilipin 1 in glucose homeostasis, we performed glucose tolerance test in Plin1 KO mice and WT mice. We discovered that the glucose responsiveness was more sensitive in Plin1 KO mice compared with WT mice under normal chow diet condition. Moreover, insulin downstream signalings were altered in metabolic tissues of Plin1 KO mice. Together, these data suggest that perilipin 1 would be involved in systemic glucose metabolism, at least in part, through insulin signaling. This work was supported by National Creative Research Initiative Program (2011‐0018312) funded by the ministry of Education, Science, and Technology (MEST). J. H. Sohn and J. S. Han were supported by the BK21 program, Korea.