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NuSAP Stabilizes Kinetochore Microtubules by Attenuating MCAK Depolymerization
Author(s) -
Li Chenyu,
Liou YihCherng
Publication year - 2015
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.29.1_supplement.884.7
Subject(s) - microtubule , microbiology and biotechnology , kinetochore , chemistry , depolymerization , biology , biochemistry , chromosome , gene , organic chemistry
NuSAP functions as a microtubule stabilizer. In this study, we dissect the function of NuSAP in kinetochore microtubules and identify MCAK as a novel binding partner of NuSAP. NuSAP tightly regulates the localization and depolymerization activity of MCAK. Moreover, we demonstrate that Aurora B significantly enhances the interaction of NuSAP with MCAK and modulates the effects of NuSAP on the localization and depolymerization activity of MCAK. Our results elucidate an underlying mechanism in which NuSAP controls kinetochore microtubule dynamics spatially and temporally by modulating the localization and function of MCAK, in an Aurora B kinase‐dependent manner.