Premium
Drosophila melanogaster with a glycolytic enzymopathy exhibit altered circadian patterns but not altered egg production and fertilization levels
Author(s) -
Hollingsworth Rachel,
Finver Ethan,
Stonebraker Jonathan,
Yacobucci Kathryn
Publication year - 2015
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.29.1_supplement.884.43
Subject(s) - biology , glycolysis , fecundity , circadian rhythm , human fertilization , drosophila melanogaster , circadian clock , endocrinology , medicine , metabolism , microbiology and biotechnology , biochemistry , genetics , population , environmental health , gene
Triosephosphate Isomerase (TPI) is a glycolytic enzyme. Mutations in this enzyme are associated with a neurodegenerative disorder called TPI Deficiency. This study utilizes the TPI sugarkill flies as a glycolytic deficient model organism to observe the effects of reduced glycolysis on fertility, fecundity and developmental timing. Fertilization requires mating, which is both controlled by neurological function and energy production by the fly to support the activity. Egg production has been shown to be dependent upon sufficient nutrients in the diet and on lipid metabolism. Therefore, it was hypothesized that altered metabolic activity in TPI sugarkill homozygotes may result in lowered fecundity and fertilization rates when compared to a wildtype control. Furthermore, as circadian control of egg laying and eclosion depend upon neurological components timing of these events can be expected in the glycolytic mutant that exhibits other neurological defects in regulation of circadian timing. The results of the study suggest that circadian timing of egg laying and eclosion is altered in the TPI sugarkill flies, but the number and quality of the eggs is not affected by the reduced glycolytic activity.