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Large‐scale integrated microfluidics for protein modification discovery in quasi‐cellular environments
Author(s) -
Tzur Amit,
Noach Meirav,
Nevenzal Hadas,
Glick Yair,
Chorni Evelin,
Avrahami Dorit,
BarbiroMichaely Efrat,
Gerber Doron
Publication year - 2015
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.29.1_supplement.884.38
Subject(s) - microfluidics , modular design , flexibility (engineering) , computational biology , biomarker , nanotechnology , posttranslational modification , ubiquitin , biomarker discovery , computer science , chemistry , biology , biochemistry , materials science , proteomics , enzyme , statistics , mathematics , gene , operating system
We introduce a modular microfluidic platform for monitoring multiple post‐translational modifications of freshly synthesized protein arrays. The system's potency, specificity and flexibility is demonstrated for tyrosine phosphorylation and ubiquitination in quasi‐cellular environments recapitulating human cell stoichiometry. Unlimited by design and protein composition, and relying on minute amounts of biological material and cost‐effective technology, this unique approach is applicable for a broad range of basic, biomedical and biomarker research.