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Morphological Alteration of Murine MC3T3‐E1 Osteoblastic cells Induced by Inorganic Polyphosphate
Author(s) -
Tsutsumi Kaori,
Saito Nagahito,
Kawazoe Yumi,
Ooi HongKean,
Shiba Toshikazu
Publication year - 2015
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.29.1_supplement.884.32
Subject(s) - polyphosphate , golgi apparatus , ultrastructure , endoplasmic reticulum , microbiology and biotechnology , chemistry , cell culture , in vitro , biology , biochemistry , anatomy , phosphate , genetics
Inorganic polyphosphate [poly(P)] induces bone cell differentiation in vitro and in vivo; therefore, we examined morphological changes of MC3T3‐E1 osteoblastic cells cultured in the presence of poly(P). MC3T3‐E1 cells, which are immature murine bone cells, were cultured with or without poly(P) and then examined by light microscopy and electron microscopy. Ultrastructural observations revealed that MC3T3‐E1 cells had morphological characteristics of osteoblasts. Cells cultured with poly(P) were strongly stained with an anti‐collagen type I antibody, whereas cells cultured without poly(P) were not. Ultrastructural analysis of cells cultured with poly(P) revealed a well‐developed Golgi apparatus, swollen and elongated rough endoplasmic reticulum, large mitochondria, and many coated pits. MC3T3‐E1 cells were transformed from a resting phase into an active blastic phase when cultured in the presence of poly(P), which implies that poly(P) can effectively induce bone regeneration. MC3T3‐E1 cells exhibiting osteoblastic characteristics in the resting stage can be transformed into the maturating or activating phase by culture with poly(P).