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Palmitoleic Acid (C16:1n7) Treatment Enhances Fatty Acid Oxidation and Oxygen Consumption in White Adipocytes
Author(s) -
AlonsoVale Maria Isabel,
Cruz Maysa,
BolsoniLopes Andressa,
Sá Roberta,
Andrade Paula
Publication year - 2015
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.29.1_supplement.884.25
Subject(s) - palmitoleic acid , chemistry , oxygen , beta oxidation , fatty acid , food science , consumption (sociology) , biochemistry , white (mutation) , palmitic acid , organic chemistry , gene , art , aesthetics
Palmitoleic acid is a monounsaturated n‐7 fatty acid (16:1n7), produced and released by adipocytes, that has been shown to enhance whole body glucose disposal, to attenuate high‐fat‐fed mice hepatic steatosis, to protect pancreatic beta‐cells from palmitic acid‐induced death and to improve circulating lipid profile in both rodents and humans. Our group has recently found strong evidence that 16:1n7 is an important positive modulator of white adipocyte lipolysis and the content of the major lipases ATGL and HSL through a PPAR alpha‐dependent mechanism in vitro and in vivo. To study the correlation of the previously described effects of 16:1n7 in white adipose tissue with mitochondrial function, we performed oxygen consumption experiments using the OROBOROS Oxygraph‐2k. Our results show that both acute and chronic treatments with 16:1n7 enhanced basal oxygen consumption in 3T3‐L1 adipocytes by 7.6% and 12.8%, respectively. Experiments were also carried out to test whether lipolysis and respiration enhancement by palmitoleic acid are linked to improved mitochondrial fatty acid oxidation (FAO) and/or uncoupling. We observed an increase (~30%) in FAO by the adipocytes treated with C16:1n7. Taken together, our data suggest that the palmitoleic acid, by concerted action of stimulated lipolysis, mitochondrial FAO and oxygen consumption may contribute to enhance white adipocytes energy expenditure.