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Analyzing the Metabolic Microenvironment of Co‐cultured Stem Cells and Cardiomyocytes
Author(s) -
Ferng Alice,
Medina Annalisa,
Schipper David,
Connell Alana,
Penick Kitsie,
Khalpey Zain
Publication year - 2015
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.29.1_supplement.884.24
Subject(s) - stem cell , microbiology and biotechnology , progenitor cell , cell culture , cell , cell type , decellularization , biology , induced pluripotent stem cell , stem cell therapy , mesenchymal stem cell , chemistry , embryonic stem cell , biochemistry , genetics , gene , extracellular matrix
While there exists precedence over the last decade of decellularization of rodent, porcine, and human hearts to create a bioartificial heart scaffold, there remains a lot of work to be done regarding understanding the cell metabolism and bioenergetics of recellularization. Examining the metabolic demands of various progenitor/stem cell and primary cell types cultured homogenously and in co‐cultures allow for an understanding of what nutrients, substrates, growth factors, and other factors are required and/or preferred in the microenvironment of each cell type. In our study, we have compared populations of human adipose‐derived stem cells (ASCs), human amniotic fluid‐derived stem cells (AFSCs), and rat cardiomyocytes (H9C2s) all individually and in co‐culture experiments, and furthermore by varying culture media conditions. Our findings show that not only does there appear to be a difference between the stem cell types in terms of their basal mitochondrial respiration, spare respiratory capacity, and ATP production rates, but there is also a difference in their glycolytic capacity and reserve. Moreover, when ASCs or AFSCs are co‐cultured with a terminal cell line, H9C2s, differences can be seen at different cell densities (15K to 80K cells/well). Changes in coupling efficiency and other parameters are seen after cell densities have been increased, and the ratios of stem cells to cardiomyocytes (30:70, 50:50, or 70:30) also affects cell metabolism. A better understanding of metabolic differences in a stem cell‐cell microenvironment may additionally determine its credibility in clinical stem cell therapy applications.