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Screening and Characterization of Hyper Jasmonic acid Producing Mutant of Lasiodiplodia theobromae
Author(s) -
Patel Disha,
Thakkar Vasudev
Publication year - 2015
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.29.1_supplement.884.22
Subject(s) - jasmonic acid , lasiodiplodia theobromae , mutant , biochemistry , biology , chemistry , pathogenic fungus , food science , botany , gene
Lasiodiplodia theobromae , a plant pathogenic fungus is well known for the production of jasmonic acid (JA) since 1971. Jasmonic acid is of importance in perfume industries, in preparation of toilet soap, chewing gum and cigarettes. Now a day's JA is used as a signalling molecule to improve crop yield. Keeping in view its industrial importance, hyper jasmonic acid producing mutants of L. theobromae were generated using ethylmethanesulfonate and characterized. Two mutants having capacity to produce hyper jasmonic acid 70 mg/lit b201 and 78 mg/ lit d103 compared to wild type 32 mg/lit strain were selected. Lipoxygenase activity and dry mycelial weight on 3 rd to 8 th day of growth were measured and it was found to be higher in both hyper jasmonic acid producing mutant. Total protein profiling was done and some protein bands were found to be induced in d103, b201 compared to wild. After 2D‐ gel electrophoresis of total protein of d103 it was found that 5',5'''‐P‐1,P‐4‐tetraphosphate phosphorylase 2 that is related to nucleotide metabolism and one hypothetical protein were over expressed in b201 mutant. Both the mutant strains were found to be useful for the higher jasmonic acid production, which could be further utilized industrially.