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Transient Expression of Intrinsically Disordered Proteins Fuels the Emergence of Adaptive Protein‐based Heritable Traits
Author(s) -
Jarosz Daniel
Publication year - 2015
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.29.1_supplement.882.15
Subject(s) - biology , phenotype , inheritance (genetic algorithm) , genetics , intrinsically disordered proteins , yeast , non mendelian inheritance , open reading frame , heat shock protein , function (biology) , gene , microbiology and biotechnology , computational biology , biochemistry , peptide sequence , mitochondrial dna
Punctuated burst of protein expression are common in biological systems. Yet whether this has a lasting influence on future generations is largely unknown. To investigate this question we transiently overexpressed virtually all yeast open reading frames. Expression of many intrinsically disordered proteins (IDPs) created heritable new traits that persisted after protein overproduction was stopped. The inheritance patterns of these traits resembled protein‐based genetic elements, known as prions, but did not depend upon the Hsp104 disaggregase. Instead, transmission from one generation to the next required molecular chaperones (Hsp70 or Hsp90). Moreover, these IDPs lacked Q/N‐rich sequences and did not form amyloid fibers. Several conferred gain‐of‐function phenotypes, and most improved growth in a wide variety of environmental stresses. Such protein‐based inheritance was also common in wild yeast strains. Thus, IDPs can drive multiple mechanisms of protein‐based inheritance, each of which broadly governs adaptive traits in nature.