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Post‐pneumonectomy Lung Deformation is Associated with Alveolar Type II Cell Apoptosis and Altered Parenchymal Mechanics
Author(s) -
Ysasi Alexandra,
Gibney Barry,
Bennett Robert,
Chamoto Kenji,
Ackermann Maximilian,
Tsuda Akira,
Konerding Moritz,
Mentzer Steven
Publication year - 2015
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.29.1_supplement.876.11
Subject(s) - lung , parenchyma , medicine , pneumonectomy , phrenic nerve , pathology , cardiology , anatomy , respiratory system
Murine pneumonectomy (PNX) is an established model of adult compensatory lung growth. After PNX, there is significant deformation of the right lung. To determine if the early mechanical events after PNX could be a stimulus to regenerative growth, we examined the peripheral parenchymal mechanics and cell population dynamics of the right lung within 3 days after PNX. Lung deformation after 1) PNX alone, 2) PNX + phrenic nerve transection (PNT) and 3) PNX + plombage was evaluated using 3D reconstructions of MicroCT scans. Deformation after PNX alone was greater than either PNX+PNT or PNX + plombage mice (displacement 5.2+‐0.8mm; p<0.01). Parenchymal mechanics, evaluated by forced oscillation, demonstrated a peak in tissue damping (G=7.01, p<0.01) and a decrease in lung compliance (C=0.03, p<0.05) coincident with the peak of lung deformation. The mechanical changes were blunted by phrenic nerve transection and plombage. Flow cytometry 24 hours after surgery demonstrated the number of alveolar Type II cells decreased by 48% in the maximally deformed lobe (cardiac) (p=0.06) and 35% in the remaining right lung (p<0.05). Coincident with the decline in the number of Type II cells, there was an increase in apoptotic Type II cells. The association of post‐PNX lung deformation and increased parenchymal stiffness with alveolar Type II cell apoptosis suggests that altered surface forces are an early event after PNX and may play a role in regenerative growth.