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Lipopolysaccharide(LPS) Exposure During The Early Postnatal Period Reduces The Volume of Type II Cells in The Developing Rat Carotid Body
Author(s) -
Master Zankhana,
Mason Ariel,
Porzionato Andrea,
Shirahata Machiko,
Gauda Estella
Publication year - 2015
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.29.1_supplement.861.9
Subject(s) - lipopolysaccharide , carotid body , saline , medicine , inflammation , endocrinology , immunohistochemistry , microglia , chemistry , andrology , carotid arteries
The carotid body (CB) contains type I(chemoreceptive) and II(supportive) cells, nerve fibres, blood vessels and connective tissues. The rat CB matures in the first two weeks of life, and we recently reported in a rat model of inflammation that hypoxic chemosenstivity of the carotid sinus nerve is attenuated in newborn rats exposed to LPS. Thus, we hypothesized that early postnatal exposure to inflammation leads to morphological changes in developing rat CB. At postnatal day 2 (P2) pups where adminstered LPS(100ug/kg) or saline(SAL) intraperitoneally. At P9‐10, one week after exposure, CBs were collected, processed and evaluated with Cavalieri's method for 1) changes in CB volumes, and 2) changes in volume components occupied by Type I and II cells by immunohistochemistry with anti‐TH and anti‐S‐100 antibodies, respectively. Sal group had slightly larger CBs (0.017±0.003 mm3, mean±SD) compared to LPS (0.013±0.003 mm3). While the volume of TH+ component was the same between the treatment groups, the volume of S100+ component was 1.5x less in LPS vs SAL animals(LPS 1.6670*10^6 ± 0.3108*10^6 µm3 vs Sal 2.3090*10^6 ± 0.536*10^6 µm3; Mann‐Whitney test, p=0.04). In conclusion, LPS exposure during early postnatal period reduces the number of type II cells in the CB. Since type II can differentiate into type I cells, and participate in co‐transmission of hypoxic signaling; a decrease pool of type II cells may adversly effect development and function of the CB leading to breathing disorders.