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Carotid Body Denervation Markedly Improves the Survival of Monocrotaline Induced Pulmonary Hypertension Rats
Author(s) -
Shinoda Masako,
Saku Keita,
Abe Kohtaro,
Takehara Takako,
Kuwabara Yukimitu,
Yoshida Keimei,
Fujii Kana,
Nishizaki Akiko,
Oga Yasuhiro,
Sunagawa Kenji
Publication year - 2015
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.29.1_supplement.858.3
Subject(s) - medicine , carotid body , ventricle , heart rate , denervation , pulmonary hypertension , endocrinology , heart failure , blood pressure , cardiology , anesthesia , carotid arteries
BACKGROUND Enhanced sympathetic nerve activity (SNA) plays a pivotal role in the pathogenesis of pulmonary hypertension (PH). Since carotid body denervation (CBD) inhibits excessive sympatho‐excitation in animal models of heart failure, we hypothesizes that CBD reduces SNA and improves survival in monocrotaline (MCT) induced PH rats. METHODS: In 5 week‐old Sprague‐Dawley rats, we removed the tissues around the carotid bifurcation and established CBD. A week after CBD, we injected MCT subcutaneously (60 mg/kg). Four weeks after MCT, we compared physiological findings and the survival rate between SHAM (CBD‐/MCT+, n=10) and CBD (CBD+/MCT+, n=13). RESULTS: CBD markedly improved the survival rate (53 vs. 100%, p=0.01, Fig.1). At 4 weeks after MCT, CBD significantly increased heart rate variability (4.3±1.3 vs. 9.0±2.1 ms, p<0.05) whereas decreased 24‐hours urinary norepinephrine (0.75 vs. 0.50 μg/day, p=0.08) suggesting that CBD reduced SNA and restored autonomic function. Interestingly, CBD did not change either right ventricular systolic pressure (59±17 vs. 48±20 mmHg) or right ventricle weight (1.4±0.27 vs 1.2±0.28 g/kg).Conclusion CBD restored autonomic balance and improved the survival rate in the MCT induced PH rats. The central mechanism that benefits the PH rats remains to be seen.