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Effects of Quercetin on L‐Arginine‐Induced Acute Pancreatitis in Rats
Author(s) -
Rotimi Solomon,
Rotimi Oluwakemi,
IyandaJoel Wisdom,
Adelani Isaacson,
Oguamanam Nnenna,
Ohamobi Onyinye
Publication year - 2015
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.29.1_supplement.856.4
Subject(s) - quercetin , arginine , lipid peroxidation , glutathione peroxidase , chemistry , glutathione , endocrinology , superoxide dismutase , acute pancreatitis , medicine , antioxidant , amylase , glutathione reductase , lipase , myeloperoxidase , biochemistry , enzyme , inflammation , amino acid
This study evaluated the effect of quercetin on oxidative stress in a rat model of L‐arginine‐induced acute pancreatitis. Thirty male rats were randomly divided into five experimental groups thus: control, L‐arginine group (2g/Kg body weight, i.p), and other groups were treated with 12.5mg/Kg body weight, 25mg/Kg body weight and 50mg/Kg body weight an hour after L‐argnine administration. Twenty four hours thereafter, the rats were sacrificed and blood collected by cardiac puncture and organs were excised for the assay of plasma lipase and α‐amylase activities as well as the activities of some antioxidant enzymes and levels of reduced glutathione, lipid peroxidation and chloramine. Acute pancreatitis was assessed by a significantly (p<0.05) increase in the activities of plasma lipase and α‐amylase 24hours after L‐arginine administration. All the quercetin dosages significantly (p<0.05) reversed the activities of these enzymes. L‐arginine administration resulted in significant (p<0.05) reduction in the activity of glutathione‐s‐transferase in the lungs, pancreas and spleen as well as in the level of erythrocyte reduced glutathione. Only rats treated with 50mg/kg quercetin had a significant (p<0.05) reversal. However, all the quercetin treated groups had significant (p<0.05) increase in the level of erythrocyte reduced glutathione. Superoxide dismutase and peroxidase activities significantly (p<0.05) reduced while myeloperoxidase activity significantly (p<0.05) increased in the organs of rats as a result of L‐arginine administration. These alterations were prevented by quercetin. These results show that quercetin protects the rat tissues from oxidative damage in L‐arginine‐induced pancreatitis.