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Atrial Natriuretic Factor (ANF) is Synthetized in the Rat Exocrine Pancreas
Author(s) -
Najenson Ana,
Bianchi Mariana,
Ventimiglia Maria,
Rodriguez Myrian,
Casco Victor,
Vatta Marcelo,
Bianciotti Liliana
Publication year - 2015
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.29.1_supplement.856.1
Subject(s) - medicine , exocrine pancreas , endocrinology , pancreas , chemistry
In previous studies we showed that ANF regulates the exocrine pancreas function in the rat through NRP‐C receptors ( Am J Physiol Gastroinetest Liver Physiol. , 285:G929, 2003; 292: G349, 2007: Gastroenterology, 149:1292, 2011). We also showed that the rat pancreas expresses the three natriuretic peptide receptors mRNAs ( Am J Physiol Gastroinetest Liver Physiol ., 293: G987, 2007). Although the heart is the major source of ANF, extracardiac sites like the gastrointestinal tract and salivary glands have been reported. In the present study we aimed to determine whether ANF is produced in the exocrine pancreas. Sprague‐Dawley rats (180‐200 g) fasted overnight were used. Following euthanasia, pancreas was harvested for immunoblotting, immunofluorescence and RT‐PCR assays. Results showed that mRNA ANF was present in the rat pancreas and further the peptide was evidenced at the protein level. Immnunofluorescence assays revealed the presence of ANF in pancreatic acinar cells. Present findings support that ANF is produced in the exocrine pancreas and further suggest that the atrial peptide may act in a paracrine/autocrine manner on pancreatic cells once released.