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Mechanisms of Alteration of Epithelial Cell by Associated Adherent‐Invasive E. coli
Author(s) -
Iaquinto Gaetano,
Marano Angela,
Perna Angelica,
Rotondi Aufiero Vera,
Mazzarella Giuseppe,
De Luca Antonio
Publication year - 2015
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.29.1_supplement.854.14
Subject(s) - escherichia coli , biology , cell cycle , microbiology and biotechnology , gene , bacteria , cell , pathogenic escherichia coli , gene expression , genetics
Increased numbers of mucosa‐associated Escherichia coli are observed in Crohn's disease (CD) and ulcerative colitis (UC). In particular the patients affected by those diseases are abnormally colonized by pathogenic adherent‐invasive Escherichia coli (AIEC). In the present study, using an in vitro model with Caco2 cells, we have studied the effect on the cell cycle of two strains belonging to Adherent‐Invasive Escherichia coli (AIEC): LF82 and O83:H1. Caco2 cells were infected with approximately 20 bacteria per cell and after 3 hours they were analyzed by the FACS. We observed that for both strains there is a block of the cell cycle into S phase, assuming DNA damage, as confirmed by DAPI staining. To allow for bacteria pathogenicity and persistence, bacteria have developed mechanisms that modify expression of host genes involved in cell cycle progression, apoptosis, differentiation and the immune response. Probably the effect of this is given by epigenetic changes in the host genome. Finally, by real time technique we observed that the expression of DNMT1 (de novo methyltransferase) gene and MGMT (DNA repair gene) were modulated. In particular we saw an increase of the expression of DNMT1 and a reduction in the levels of MGMT in Caco2 cells infected with LF82 and O83:H1.