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Lactobacillus rhamnosus Strain GG Influences on the Longevity and Immune Response of Caenorhabditis elegans to Foodborne Pathogens with Stimulating MicroRNAs.
Author(s) -
Oh Sangnam,
Park Mi Ri,
Son Seok Jun,
Kim Younghoon
Publication year - 2015
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.29.1_supplement.853.3
Subject(s) - lactobacillus rhamnosus , caenorhabditis elegans , biology , probiotic , microbiology and biotechnology , pathogen , immune system , in vivo , bacteria , nematode , longevity , strain (injury) , lactobacillus , innate immune system , gene , immunology , genetics , anatomy , ecology
The immune response of Caenorhabditis elegans , surrogate in vivo host model, to pathogen infections is well‐established in wide ranges of biotechnologies. However, very limited and few information has been discovered about the impacts of health promoting‐probiotic bacteria on host responses. Here we investigated the potential probiotic activity of Lactobacillus rhamnosus strain GG on in vivo host using C. elegans nematode model. Initially, we determined the survivals of C. elegans using solid‐killing assay and examined the bacterial colonization assay in C. elegans gut by plate counting and transmission electron microscopy (TEM). Our results showed that the probiotic L. rhamnosus strain GG was not harmful to C. elegans and colonized in the C. elegans intestine compared to normal fed C. elegans with bacterium E. coli OP50 as control. Moreover, persistent L. rhamnosus strain GG in the nematode intestine significantly enhanced the resistance of nematodes exposed to both Gram‐ positive and ‐ negative pathogens as well as prolonged the lifespan of nematodes. We performed microRNAs microarray whether microRNAs are involved in prolonged longevity of C. elegans . Conditioning of L. rhamnosus strain GG to C. elegans highly regulated a number of microRNAs including mir‐34, mir‐43, and miR‐239, which are significantly related to aging and immune modulation. In conclusion, probiotic conditioning with L. rhamnosus strain GG enhanced the longevity and resistance to foodborne pathogen infections through stimulating microRNAs in C. elegans in vivo host.