Premium
Hepatic steatosis following loss or gain in body mass
Author(s) -
Mendes Iara Karise,
Martins Marcela,
Mury Wanda,
Àguila Marcia,
Brunini Tatiane
Publication year - 2015
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.29.1_supplement.846.6
Subject(s) - steatosis , medicine , endocrinology , chemistry , nonalcoholic fatty liver disease , fatty liver , disease
Nonalcoholic fatty liver disease is emerging as the most common chronic liver condition in the Western world, and it is closely related to obesity.Here,we investigated the impact of changes in body mass on metabolic parameters and hepatic steatosis in an animal .Male C57BL/6 mice were divided into four groups (n=10):SC, standard chow for 16 wk,; HF, high fat diet for 16 wk; HF‐SC, HF diet for 8 wk followed by SC for 8 wk; and SC‐HF, SC for 8 wk followed by HF diet for 8 wk. At the end of study, animals from HF group presented a greater body mass than the other groups. In SC‐HF, a significant increase in body mass was seen after diet exchange; and the opposite was seen in HF‐SC, in which body mass values resembled the values of SC group at week 16. Serum levels of total cholesterol were higher in HF and HF‐SC than in SC and HF‐SC (SC:3.5±0.2, HF:5.8±0.2; SC‐HF:4.6±0.1; HF‐SC:3.4±0.3 mmol/L). HF group presented higher fasting glucose levels than the other groups (SC:9.0±0.7; HF:15.6±0.5; SC‐HF:11.2±0.5; SC‐HF:11.6±3.2 mmol/L).HF animals showed an impaired glucose tolerance, assessed by an oral glucose tolerance test, compared to the other groups (SC:1104±36; HF:1481±112; SC‐HF:1095±28, HF‐SC:956±10 arbitrary units). Hepatic steatosis was quantified by stereology. Higher amounts of steatosis were observed in HF compared to the other three groups. The HF‐SC and SC‐HF groups exhibit increased steatosis compared to SC, but reduced levels compared to HF group. HF‐SC group showed a lower amount of steatosis that the SC‐HF group. Our findings demonstrate that diet‐induced body mass loss is an important strategy to reduce intra‐hepatic lipid accumulation and to improve its adverse metabolic effects.