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Higher than Equilibrium Intracellular Cl ‐ Concentration in Dorsal Root Ganglion Neurons of NKCC1 Knockout Mice
Author(s) -
AlvarezLeefmans Francisco Javier,
Mao Shihong
Publication year - 2015
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.29.1_supplement.845.22
Subject(s) - dorsal root ganglion , intracellular , chemistry , cotransporter , biophysics , basal (medicine) , dorsum , endocrinology , anatomy , biochemistry , biology , sodium , organic chemistry , insulin
It is well established that intracellular Cl ‐ concentration [Cl ‐ ] i in dorsal root ganglion (DRG) neurons is maintained above electrochemical equilibrium via Na + K + 2Cl ‐ cotransporter NKCC1. To determine if NKCC1 is the only mechanism of active accumulation of Cl ‐ in primary sensory neurons, we measured [Cl ‐ ] i in freshly dissociated DRG neurons of NKCC1 knockout (KO) and wild‐type (WT) mice (p5 to p21), using MQAE and fluorescence imaging microscopy. The basal [Cl ‐ ] i in 54 WT neurons was 35.7 ± 18 (SD) mM i.e. two to three times higher than expected for a passive distribution. The corresponding Cl ‐ equilibrium potential (E Cl ) was ‐36.6 ± 13 mV. The basal [Cl ‐ ] i in NKCC1 KO neurons (n=121) was 31.6 ± 17.4 mM, and the corresponding E Cl was ‐40.4 ± 14.5 mV. The differences between means of WT and KO [Cl ‐ ] i and E Cl were not statistically significant. These results suggest that NKCC1 is not the only active transport mechanism maintaining intracellular Cl ‐ above equilibrium in DRG neurons. Whether these additional transport mechanisms are compensatory or exist in WT neurons, remains to be determined.