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The Role of Voltage‐gated Potassium Channels in Osteogenic Differentiation
Author(s) -
Yang Ji Eun,
Song Min Seok,
Ryu Pan Dong,
Lee So Yeong
Publication year - 2015
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.29.1_supplement.845.16
Subject(s) - microbiology and biotechnology , mesenchymal stem cell , cellular differentiation , chemistry , osteoblast , potassium channel , cell culture , voltage gated potassium channel , channel blocker , potassium channel blocker , cell growth , endocrinology , biology , in vitro , biochemistry , gene , genetics , organic chemistry , calcium
Voltage‐gated potassium (Kv) channels are known regulators of cellular electrical properties in excitable cells such as neurons and muscle cells. The Kv channels also play roles in cell proliferation, differentiation and cell survival in non‐excitable cells. In the present study, we focused on the role of Kv channels in osteogenic differentiation using human mesenchymal stem cells (hMSCs) and the osteoblast‐like MG‐63 and SaOS‐2 cell lines.
Use of a non‐specific Kv channel blocker, tetraethylammonium (TEA), confirmed that blockade of Kv channels in hMSCs increased their mineral contents density, an indicator of osteogenic differentiation. We also used two osteoblast‐like cell lines, MG‐63 and SaOS‐2 cells, to focus on the regulation of osteogenic properties by Kv channel blockers. We screened the mRNA and protein expression of Kv channels in MG‐63 and SaOS‐2 cells and compared the relative expression of Kv channels during osteogenic differentiation. We confirmed that the expression level of Kv7.3 was significantly changed, while blockade of Kv7.3 using the selective Kv7.3 blocker, linopirdine and XE991, increased osteogenic differentiation in MG‐63 and SaOS‐2 cells. In contrast, the use of Kv7 opener, flupirtine, caused no significant change in osteogenic differentiation in either cell line. In conclusion, Kv channels, especially the Kv7 families, are involved in osteogenic differentiation and have potential functions in hMSCs, MG‐63 and SaOS‐2 cells during osteogenic differentiation. Blockade of Kv7.3 may be important in regulation of osteogenic differentiation.