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K + secretion by ionocytes in the skin of medaka larve
Author(s) -
Yu Li,
Liu Sian,
Chen Po,
Lin Li
Publication year - 2015
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.29.1_supplement.843.4
Subject(s) - oryzias , gill , secretion , zebrafish , chemistry , potassium , sodium , microbiology and biotechnology , medicine , biochemistry , fish <actinopterygii> , biology , gene , organic chemistry , fishery
Epithelial ionocytes in fish gills and skin play a critical role in ionic and acid‐base regulation. Molecular mechanisms of sodium, chloride and acid‐base regulation in fish ionocytes have been well investigated. However, the regulatory mechanism of potassium, which is a major monovalent cation in fish, is still unclear. In a recent study, a sub‐group of ionocyte, which expressed potassium channels (ROMK) at the apical membrane was identified in zebrafish and Mozambique tilapia. In this study, we further investigated the mechanism of K+ secretion by ionocyte in medaka (Oryzias latipes). With a scanning ion‐selective electrode technique (SIET), K+ secretion was detected at the apical surface of a group of ionocyte in medaka larvae. Quantitative PCR showed that mRNA level of ROMK was induced in fish exposed to high K+ water. Loss‐of‐function studies showed that the K+ secretion was via ROMK. Taken together, this study suggests that the ROMK‐expressed ionocyte is responsible for K+ secretion in medaka.