Premium
Stereotaxic Coordinates of the Brain of Guinea Pigs
Author(s) -
Nakasone Alice,
Tsai HsiuWen Irene,
Davenport Paul
Publication year - 2015
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.29.1_supplement.841.3
Subject(s) - coronal plane , amygdala , cresyl violet , anatomy , sagittal plane , neuroscience , medicine , biology , pathology , staining
Although much research is done with a mouse model, a guinea pig model is preferentially used for cough stimulation experiments because a mouse is unable to cough. Currently, there is no literature that provides stereotaxic coordinates of the guinea pig brain. The purpose of this project was to study stereotaxic coordinates for future experiments, specifically for locating regions such as the amygdala, periaqueductal gray (PAG), and nucleus tractus solitarius (NTS). The amygdala is part of the limbic system and is involved in the recognition of our emotions and motivations. PAG is involved in homeostatic regulation of salient functions such as pain, anxiety and autonomic function. NTS is the site of integrating and processing peripheral afferents such as respiratory and cardiovascular afferents. The guinea pigs were sacrificed and their brains were harvested for histological analysis. The fixed brains were coronally and sagittally sectioned into 40 mm thick slices with a microtome for cresyl violet staining. The obex was used as a rostrocaudal zero reference point for the coronal sections and the midline was used as a dorsoventral zero reference point for sagittal sections. The results showed the stereotaxic coordinates for amygdala (coronal: +1.90 to +2.388 cm; sagittal: +0.488 cm), PAG (coronal: +1.636 to +2.004 cm; sagittal: +0.248 cm), and NTS (coronal: ‐0.008 to +0.408 cm; sagittal: +0.248 cm). Results continue to define the locations of the amygdala, PAG and NTS in guinea pigs for multi‐electrode array recording during swallow and cough stimuli. (NIH – Short Term Training, University of Florida #83224).