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Calycosin may improve outcomes of ischemic stroke in rats by inhibiting activated microglia in brain
Author(s) -
Kuo TingWei,
Chang ChingPing
Publication year - 2015
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.29.1_supplement.835.4
Subject(s) - calycosin , microglia , medicine , ischemia , neuroprotection , pharmacology , stroke (engine) , brain ischemia , middle cerebral artery , anesthesia , inflammation , mechanical engineering , daidzein , formononetin , genistein , engineering
Calycosin, an isoflavonoid, is the major active component in Radix Astragali . It has a neuroprotective effect against cerebral ischemia/reperfusion in rats. The mechanisms remain unclear. This study aimed to assess whether calycosin improves outcomes of ischemic stroke by affecting activated microglia formation. After 1 h of reperfusion following ischemia for 60 minutes induced by middle cerebral artery occlusion (MCAO), Sprague‐Dawley rats were intragastrically administered a dose of 30 mg/kg. Then, an i.g. dose of 30 mg/kg of calycosin was administered daily for consecutive 7 days. Neurological deficit, infarct volume, histopatholical and immunofluorescence staining were evaluated after 7 days of reperfusion. Treatment with calycosin significantly ameliorated neurological deficit and infarct volume after cerebral ischemia reperfusion. Calycosin also attenuated MCAO‐induced activated microglia (evidenced by the increased numbers of both the amoeboid microglia and the cells colocalized with microglia and tumor necrosis factor‐alpha‐specific markers). Our results suggest that calycoin may improve outcomes of ischemic stroke in rats by inhibiting activated microglia in brain.