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Central action of Insulin on the Modulation of Autonomic Balance to Control Hepatic Glucose of Wistar and SHR: Any Interaction Between Hypothalamic Paraventricular Nucleus and Dorsal Vagal Complex?
Author(s) -
Ribeiro Izabela,
FerreiraNeto Hildebrando,
Canteras Newton,
Antunes Vagner
Publication year - 2015
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.29.1_supplement.828.7
Subject(s) - endocrinology , medicine , insulin , phentolamine , vagus nerve , propranolol , splanchnic nerves , splanchnic , atropine , autonomic nervous system , stimulation , heart rate , blood pressure , hemodynamics
Insulin is an important to control of glucose level, but little is known about its action on modulation of autonomic nervous system (ANS) to control of glicemia, especially in hypertension. Here, Wistar and SHR had ICV injection of insulin (100 nU/µL) and hepatic glucose (HG) decreased by 27% in Wistar, with no change (3%) in SHR. Pretreatment with atropine blocked the fall in the HG, while propranolol + phentolamine attenuated the decreasing (8%) in HG after insulin ICV in Wistar rats. An intracarotid infusion of insulin was able to increase the subdiaphragmatic vagus nerve (SVN) activity in Wistar (12±2%) with no changes in the lumbar splanchnic sympathetic nerve (LSSN) activity (‐6±3%). No change was observed in SVN and LSSN (‐2±2% and 2±3%, respectively) in SHR. Wistar rats were subjected to injection of Fluorogold (FG) in the DMV, and 10 days after received insulin for analysis of FOS expression. An increase in the Fos labeled cells was found in the PVN of insulin treated rats compared to the control group (412±44 vs 132±53 cells/mm 2 ). In the cases of the FG injection in the DMV, a number of FG + Fos double labeled cells were found in the PVN. In conclusion, icv insulin selectively activates parasympathetic pathways in Wistar, but not in SHR, and this effect may result from a refined integration between PVN and DMV neuronal circuitry coordinating the sympatho‐vagal balance for metabolic and cardiovascular adjustments. Financial support: FAPESP # 2013‐06206‐0; CNPQ .