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IL15 Promotes Myogenic and Brown Adipose Tissue Associated Genes while Decreasing AMPK Activation in C2C12 Skeletal Muscle Cells
Author(s) -
Abbott Marcia,
Turcotte Lorraine
Publication year - 2015
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.29.1_supplement.826.3
Subject(s) - myogenesis , ampk , myf5 , biology , myokine , endocrinology , c2c12 , skeletal muscle , medicine , microbiology and biotechnology , myogenin , phosphorylation , protein kinase a
Skeletal muscle (SKM) has come to the forefront as an endocrine regulator of obesity due to its ability to secrete myokines into circulation. Many myokines, in particular IL15, have been shown to increase in circulation following exercise. IL15 may act to decrease fat mass while increasing lean mass and possibly increase brown adipose tissue (BAT). Little is known about the molecular mediators of IL15 following exercise. We have previously shown that AMPK acts upstream of IL15 expression but data are conflicting. Here we aimed to determine the effects of IL15 on genes associated with myogenesis, BAT, and AMPK activation. Differentiated C2C12 SKM cells were stimulated for 24 hrs with 100 or 1000 ng/ml of IL15. Cells were harvested and mRNA was measured for myogenic markers Myf5, MCK, mitochondrial uncoupling protein UCP2, and genes highly expressed in BAT CIDEA and ELOVL. Additionally, phosphorylation (p) of AMPK at Thr172 was assessed by western blotting. IL15 stimulated increases (P<0.05) in Myf5, MCK, UCP2, and CIDEA mRNA expression exclusively with 100 ng/ml. Significant elevations in ELOVL mRNA expression were only observed with 1000 ng/ml of IL15 treatment (P<0.05). C2C12 cells treated with both doses of IL15 decreased pAMPK. Our data indicate that IL15 is involved in promoting myogenic, mitochondrial uncoupling, and BAT associated gene expression. Further it appears that IL15 paradoxically decreases activation of AMPK in C2C12 cells. Further studies aimed at elucidating the role of IL15 in mediating SKM metabolism, in particular myogenesis and BAT, are warranted, thus providing potential therapies for the treatment of obesity.