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Lipid‐Induced Anabolic Resistance in Skeletal Muscle is Associated with Inflammation in Aging, but Not Obesity
Author(s) -
Rivas Donato,
McDonald Devin,
Rice Nicholas,
Fielding Roger
Publication year - 2015
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.29.1_supplement.825.5
Subject(s) - anabolism , endocrinology , medicine , insulin resistance , skeletal muscle , inflammation , proinflammatory cytokine , lipid metabolism , stimulation , obesity , biology
The loss of skeletal muscle mass is observed in many pathophysiological conditions including, aging and obesity. Chronic inflammation is observed with aging and obesity may play a critical role in the muscle's resistance to anabolic stimulation. A characteristic metabolic feature of both these conditions is an increase in the accumulation of intramyocellular lipid (IMCL) content. Therefore, we tested the hypothesis that lipid‐induced anabolic resistance is mediated by inflammation in both aging and obesity. In order to test this hypothesis we used C57BL/6 mice aged 6 months and fed a control diet (YNG, 15% fat/kcal) or a high‐fat (FAT, 60% fat/kcal) and mice aged 24 months fed a control diet (OLD) for 12 wk. The accumulation of intramuscular lipids was increased in both OLD and FAT compared to YNG. The phosphorylation of anabolic signaling components was increased in all groups after insulin stimulation but differentially affected in OLD and FAT. Differences in the activation and gene expression of the NFκB proinflammatory signaling pathway were only observed in OLD, but not FAT. We conclude that the accumulation of lipids during aging and obesity have divergent effects on inflammation in skeletal muscle.