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Differential Effects of Testosterone and Trenbolone on Skeletal Muscle Markers of Ribosome Biogenesis
Author(s) -
Mobley Christopher,
Kephart Wesley,
Fox Carlton,
Santucci Vincent,
Beck Darren,
Yarrow Joshua,
McCarthy John,
Kirby Tyler,
Borst Stephen,
Roberts Michael
Publication year - 2015
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.29.1_supplement.825.21
Subject(s) - androgen , medicine , testosterone (patch) , endocrinology , messenger rna , gene expression , biology , gene , hormone , biochemistry
We sought to determine if androgen administration to orchiectomized (ORX) rats affects ribosome biogenesis markers. Ten month‐old male Fischer 344 rats (n=10‐11/group) were treated for 4 weeks following 2 weeks after ORX/Sham surgery with either: a) ORX + testosterone enanthate (ORX+Test; 7 mg/wk), or b) ORX + trenbolone enanthate (ORX+Tren; 1 mg/wk), a non‐5α‐reducible, non‐aromatizable synthetic testosterone analogue. LABC muscle was removed during necropsy and genes of interest were analyzed using RT‐PCR. A trend towards significance existed between groups regarding total RNA/mg wet muscle (p = 0.098), with ORX being 20‐23% lower versus other groups. Interestingly, several ribosomal biogenesis markers were greater in SHAM versus other groups (p < 0.001). 5S rRNA and Raptor mRNA were greater in ORX+Tren versus other groups, respectively (p < 0.05). Rpl‐3 mRNA was greater in ORX and ORX+Tren versus SHAM (p = 0.048, 0.002), and was also greater in ORX+Test (p < 0.001, 0.02) versus SHAM and ORX. Nucleophosmin mRNA was greater in ORX versus other groups (p < 0.001). ORX suppressed the expression of various ribosomal markers and genes, though androgen treatment largely rescued this effect.