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Acupuncture plus Low‐Frequency Electrical Stimulation (Acu/LFES) Attenuates Diabetic Myopathy by Enhancing Muscle Regeneration
Author(s) -
Robinson Alayna,
Klein Janet,
Wang Xiaonan
Publication year - 2015
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.29.1_supplement.825.1
Subject(s) - myogenin , myogenesis , myod , medicine , endocrinology , muscle atrophy , diabetes mellitus , skeletal muscle , protein catabolism , stimulation , biology , biochemistry , amino acid
The mortality and morbidity are increased in the patients with catabolic diseases such as diabetes. Previous, we discovered that exercise has the ability to thwart the detrimental effects caused by catabolic diseases. Unfortunately, patients with severe diseases usually are unable to consistently exercise. Therefore, we hypothesize that Acu/LFES will mimic exercise and prevent diabetes‐induced muscle loss. Streptozotocin (STZ) was used to induce diabetes in mice. The mice were then treated with Acu/LFES for 15 minutes every day for 2 weeks. Acupuncture points selected were according to the WHO Standard Acupuncture Nomenclature. The needles were connected into SDZ‐II Electronic acupuncture instrument using consistent pulse, electric frequency 20Hz, electric current 1mA. Western blot and mRNA expression were used to analyze the change of myogenesis proteins. The expressions of MyoD (proliferation marker), myogenin (differentiation marker) and eMyHC (differentiation and fusion marker) were significantly decreased in diabetic muscle vs. control muscle. The diabetes‐induced suppression of the myogenesis related proteins was reversed and these protein levels upregulated by Acu/LFES. Immunohistochemistry also provides evidence that Acu/LFES stimulates myogenesis by satellite cells migrate into myofibers within 24 hours after Acu/LFES treatment. Central nuclei are apparent at day‐2 and by day‐3 the decrease of central nuclei indicate fusion has occurred. We conclude that Acu/LFES is effectively against diabetes‐induced skeletal muscle atrophy and enhances myogenesis, in part, by stimulation of myogenesis. Funding: APS STRIDE Research Fund, NIH/NIAMS: R01 AR060268