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Circulating Levels of Key Myokines and Cytokines Immediately After Treadmill Exercise in Rats Fed Low‐fat or High‐fat Diets
Author(s) -
Stevens Kaitlyn,
Weaver Danielle,
Grainer Megan,
Bradley Adrienne,
Wilson Colin,
Ballard Tyler,
Sharma Naveen
Publication year - 2015
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.29.1_supplement.824.12
Subject(s) - medicine , endocrinology , sed , myokine , insulin , fgf21 , ampk , glycogen , leptin , chemistry , skeletal muscle , protein kinase a , kinase , obesity , fibroblast growth factor , receptor , biochemistry
A single bout of exercise (EX) can have benefits on glucose metabolism in both normal and insulin‐resistant individuals. Several plasma proteins secreted by muscle and other tissues have been demonstrated to have roles in regulating energy metabolism including irisin, brain‐derived neurotrophic factor (BDNF), and fibroblast growth factor‐21 (FGF‐21). However the effect on circulating levels of these proteins, after an acute bout of exercise in a model of dietary‐induced insulin resistance is not known. Rats (male Sprague‐Dawley, ~9‐mos old) were fed standard low‐fat diet (LFD), or a high‐fat diet (60% of calories from fat; HFD) for 2 weeks. There were 4 groups of rats: 1) LFD‐sedentary (LFD‐SED), 2) HFD‐SED, 3) LFD‐EX, or 4) HFD‐EX. On the day of the terminal experiment, rats in the EX groups ran for 30min on a treadmill at 18m/min. Immediately after the exercise bout, blood and tissue was taken from both EX and SED rats. HFD rats weighed more and had higher levels of insulin than LFD rats after 2 weeks of high‐fat feeding. EX rats of both diet groups had similarly reduced muscle glycogen and increased AMP‐activated protein kinase (AMPK) phosphorylation. There was no effect of diet or exercise on plasma levels of irisin or FGF‐21. BDNF levels were reduced in the high‐fat fed rats compared to rats fed low‐fat chow, however exercise did not alter BDNF levels. A single bout of exercise had no effect on levels of irisin, FGF‐21 or BDNF in either normal or insulin‐resistant rats, suggesting that these plasma proteins are not likely involved in the beneficial effects on glucose metabolism that occur immediately post‐exercise.