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Effects of Bisphenol A on C2C12 Myotubes In Vitro
Author(s) -
Kostrominova Tatiana
Publication year - 2015
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.29.1_supplement.817.10
Subject(s) - myogenesis , c2c12 , bisphenol a , in vitro , chemistry , biochemistry , organic chemistry , epoxy
The recent “epidemics” of obesity and type 2 diabetes observed in developed countries can be linked experimentally to the effects of environmental pollutants‐endocrine disrupting chemicals (EDCs). Bisphenol A (BPA) is an EDC extensively used in the production of plastics. BPA can act as an estrogen mimetic, binding to estrogen receptors and interfering with signaling cascades. Since skeletal muscle is one of the most important organs for glucose homeostasis in humans, abnormal lipid and glucose metabolism in skeletal muscle could contribute to the development of obesity and type 2 diabetes. Therefore understanding the mechanisms mediating the effects of BPA on skeletal muscle is critically important for development of future preventive/treatment options for humans. It is known that BPA is accumulated in skeletal muscle following exposure, but presently little is known about the effects of BPA on skeletal muscle structure and function. In this study we treated C2C12 myotubes in vitro with BPA at low physiologically‐relevant concentrations. Our experiments showed that exposure to BPA at 1nM‐1μM down‐regulated mRNA expression of adiponectin, a well‐known adipokine/myokine with insulin‐sensitizing effects, approximately 2‐fold. The mRNA expression of Perm1, a muscle‐specific protein critical for energy metabolism and contractile function was also down‐regulated approximately 2‐fold. The mRNA expression of FAB4 and LPL was increased 2‐3‐fold only at 100nM‐1μM of BPA. The expression of muscle‐specific degradation pathway proteins MURF1and MAFbx was not changed, suggesting that BPA exposure at 1nM‐1μM does not cause significant cell damage. Our data suggest that BPA exposure can affect lipid metabolism in skeletal muscle cells and contribute to the development of obesity‐related abnormalities. Support: IUSM‐NW.