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ANALYSIS OF MICRORNA‐22 ON CARDIAC HYPERTROPHY INDUCED BY HIGH FAT DIET
Author(s) -
Guedes Elaine,
Diniz Gabriela
Publication year - 2015
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.29.1_supplement.814.9
Subject(s) - medicine , endocrinology , cardiac function curve , adiponectin , calorie , muscle hypertrophy , cardiac fibrosis , heart failure , cardiac hypertrophy , leptin , insulin , obesity , insulin resistance
High fat (HF) diet induces cardiac hypertrophy and compromises myocardial function, which may lead to heart failure. Recent studies have showed that overexpression of miRNA‐22 is involved in some cardiovascular alterations such as cardiac hypertrophy. However, it is not clear whether the cardiac miRNA‐22 levels are regulated in cardiac remodeling induced by HF diet. Objective: The aim of the present study is to evaluate the HF diet effect on cardiac miR‐22 levels. Methods: Male mice (C57BL/6) were fed a normal diet (10% of calories from fat) and a HF diet (60% of calories from fat) for 10 weeks. Body weight, food and water intake were monitored weekly. Intraperitoneal glucose tolerance test (iGTT) was performed. Glucose levels and insulin, triglycerides, cholesterol, leptin and adiponectin serum levels were analyzed. Histological analysis of the hearts also were performed. Cardiac hypertrophy was evaluated by heart weight (HW) to tibia length (TL) ratio. The cardiac miRNA‐22 levels were assessed by miRNA Stem Loop RT‐PCR. Results: HF fed mice exhibited increased caloric intake, body weight and epididymal fat pad mass. HF diet also increased triglycerides, total cholesterol, glucose, insulin and leptin levels and induced glucose intolerance (P<0.05 vs control). In addition, HF diet induced cardiac hypertrophy, as evaluated by the higher HW to TL ratio, which was accompanied by enlarged cardiomyocytes and cardiac fibrosis. Interestingly, HF fed mice exhibited an increase on cardiac miRNA‐22 levels (P<0.01 vs control). Conclusions : These results demonstrate that miR‐22 is increased in cardiac hypertrophy induced by HF diet. However, gain‐ and loss‐of‐function studies are needed to investigate the possible role of the miRNA‐22 in cardiac remodeling induced by HF diet.