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Deoxycorticosterone acetate (DOCA) treatment increased blood pressure in female but not male mice under high salt diet
Author(s) -
Rouch Al,
Wallen Tony,
Fan Liming,
Waturuocha Chiedozie,
Kudo Lucia
Publication year - 2015
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.29.1_supplement.811.19
Subject(s) - medicine , endocrinology , blood pressure , placebo , saline , excretion , chemistry , urine , zoology , body weight , biology , alternative medicine , pathology
Sex differences in salt sensitivity have been suggested but further study is required. Our purpose was to investigate effects of DOCA‐treatment on blood pressure and the renal handling of sodium in mice consuming high salt. DOCA or placebo pellets of 50 mg (Innovative Research of America, Sarasota, FL) were implanted in 4 month‐old CD‐1 mice (Harlan, Inc., Indianapolis, IN) 3 days before being placed in metabolic cages where they remained for 24 days. Four groups (n=5/group) were used and included female and male placebo (FP, MP) and female and male DOCA, (FDOCA & MDOCA). Mice consumed 4% salt diet and drank 1% saline ad libitum for 24 days. Daily measurements of Na intake (Nai) and Na excretion (Nae) were used to determine Nae/Nai ratio. Mean blood pressure (MBP, mm Hg) was measured daily in all mice via the tail‐cuff method. Daily fluid intake and urine flow rates were extensive for DOCA‐treated mice (1.0 to 1.5 x body weight). MBP averaged for the 24 day period was higher in the FDOCA group (80.8±1.3) compared to all other groups: FP (72.6±1.2, p<0.001); MDOCA (77.5±0.6, p<0.05); and MP (75.9±1.3, p<0.05). MBP for the FP group was significantly lower than that of other groups and no difference in MBP existed between MDOCA and MP. Nae/Nai data were interesting in that the ratio for the FDOCA group was higher than that for the FP group (81.3±2.8 vs 72.4±3.0, respectively, p<0.05) whereas no difference occurred between the MDOCA and MP groups (82.5±1.8 vs 77.3±2.3, respectively). Results suggest that females may be more salt sensitive than males and lose their sex‐dependent protection against hypertension upon high salt consumption. Study supported by Oklahoma INBRE 8P20GM103447 Summer Research Program.