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Role of Renal Interstitial ATP in Pressure Natriuresis/Diuresis Relationship
Author(s) -
Palygin Oleg,
Evans Louise,
Staruschenko Alexander,
Cowley Allen
Publication year - 2015
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.29.1_supplement.811.16
Subject(s) - natriuresis , endocrinology , medicine , diuresis , chemistry , tubuloglomerular feedback , kidney , kaliuresis , nephron , adenosine
The purines, adenosine‐5'‐triphosphate (ATP) and derivatives, are recognized to be among the most important agents of paracrine signaling. Purinergic receptors are expressed ubiquitously in the kidney and influence several intrarenal regulatory mechanisms, including sodium and water transport along the nephron, tubuloglomerular feedback and mesangial cell transformation. However, information on the sources of interstitial ATP, the mechanisms of release and relationship to blood pressure have been hampered by the inability to measure changes in intrarenal ATP levels in vivo. Here we used amperometry biosensors to measure dynamic changes in cortical ATP concentrations in response to changes in arterial blood pressure (ABP) in anesthetized Sprague Dawley rats. For the experimental procedure, catheters were inserted in the carotid artery for the measurement of ABP the femoral vein for the infusion of BSA, and both ureters for the collection of urine. Ligatures were placed around the celiac and mesenteric arteries and distal aorta below both kidneys for the manipulation of ABP. Biosensors were implanted in the renal cortex for the assessment of interstitial ATP. Increased ABP activated diuresis/natriuresis processes and was associated with extracellular release of ATP. There was a linear correlation with every 1 mmHg increase in pressure resulting in a 50 nM increase in ATP. These studies are the first to measure renal interstitial ATP levels in a blood‐perfused kidney in vivo . The use of this novel technique has demonstrated that cortical ATP directly correlates with blood pressure and suggests that the pressure‐induced release of ATP may participate in the pressure‐natriuresis response.