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Sodium‐independent Dietary Effects on Renal Immune Cell Infiltration in Salt‐sensitive Hypertension
Author(s) -
Abais Justine,
Mattson Alexander,
Wade Brittany,
Lund Hayley,
Rudemiller Nathan,
Petrova Galina,
Mattson David
Publication year - 2015
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.29.1_supplement.811.11
Subject(s) - medicine , endocrinology , immune system , kidney , chemistry , il 2 receptor , sodium , cd8 , t cell , immunology , organic chemistry
The Dahl SS rat is a well‐established model of human salt‐sensitive hypertension and renal disease. Recently, sodium‐independent effects of diet, particularly during gestation, were shown to be important in the development of this disease phenotype. Dahl SS/JrHsdMcwiCrl (SS/Crl) rats fed a grain‐based diet (Teklad 5L2F) were less susceptible to salt‐induced blood pressure elevation and renal damage compared to SS/JrHsdMcwi (SS/Mcw) rats fed a casein‐based diet (AIN‐76A). With the known role of the immune system in hypertension, the present study characterized the immune cells infiltrating the kidney in both SS/Mcw and SS/Crl rats. Following a 3 week high salt (4.0% NaCl) challenge, SS/Crl rats displayed significantly less CD45+ leukocyte infiltration in the kidney than SS/Mcw (47.3% reduction, n=6‐7). Specifically, there were respective decreases in infiltrating CD3+ T cells, CD45R+ B cells, and CD11b/c+ antigen presenting cells by 52.4%, 77.7%, and 45.9%, in SS/Crl versus SS/Mcw. The SS/Crl immune system appears to be less activated, as demonstrated by a respective 44.8% and 66.8% reduction in CD4+CD25+ and CD8+CD25+ T cells, as well as a 54.9% decrease in CD4+CD25+FoxP3+ regulatory T cells. SS/Crl rats were also protected from salt‐induced kidney damage, indicated by attenuated urinary albumin (20±2 vs 99±16 mg/day) and protein (73±4 vs 173±32 mg/day) excretion compared to SS/Mcw (n=6). These differences between SS/Mcw and SS/Crl show that sodium‐independent dietary effects may influence the immune response and infiltration of immune cells in the kidney, ultimately impacting susceptibility to salt‐induced hypertension and renal damage. Support: DK96859, HL116264, 5T32HL007792.