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The Calcium Sensing Receptor Activation by Neomycin Decreases the Bicarbonate Flux in Renal Proximal Tubules of Wistar Rats
Author(s) -
Santos Priscilla M,
Fernandez Ricardo,
Malnic Gerhard,
Pessoa Thaissa
Publication year - 2015
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.29.1_supplement.809.5
Subject(s) - calcium sensing receptor , chemistry , endocrinology , medicine , nifedipine , reabsorption , agonist , receptor , homeostasis , calcium , extracellular , transcellular , bicarbonate , antagonist , calcium metabolism , kidney , biochemistry , biology
In the parathyroid gland, high extracellular Ca 2+ activates Calcium Sensing Receptor (CaSR) and suppresses PTH secretion.In the renal proximal tubule (PT), PTH inhibits NHE3 and CaSR expression, indicating that NHE3 is regulated by Ca 2+ homeostasis. Since 20% of the filtered Ca 2+ is reabsorbed via transcellular, CaSR activation may regulate NHE3 activity through cell Ca 2+ entry. The aim of this study was to determine the effect of CaSR activation and cell Ca 2+ entry on PT bicarbonate reabsorption (JHCO 3 ‐ ), via stationary microperfusion. To show the role of CaSR in JHCO 3 ‐ , PT were perfused with a 24mM HCO 3 ‐ and 1mM Ca 2+ solution (CTRL) in the presence of the CaSR agonist Neomycin (Neo, 200µM). Neo decreased JHCO 3 ‐ by 27.86% (vs CTRL), showing that CaSR activation may inhibit NHE3. The addition of the specific CaSR antagonist NPS2143 (NPS, 2µM) to Neo was able to abolish its effect, showing that Neo‐dependent JHCO 3 ‐ inhibition was due to CaSR activation. To determine if cell Ca 2+ entry was involved in this effect, the non‐specific TRP antagonist ruthenium red (RR, 5µM) or the specific L‐type Ca 2+ channel blocker Nifedipine (Nif, 200µM) (±Neo) were used. RR alone inhibited JHCO 3 ‐ , showing that TRP channels are endogenously important to maintain JHCO 3 ‐ . Interestingly, RR abolished the Neo‐dependent JHCO 3 ‐ inhibition indicating that TRP channels are involved in the Neo effect in an opposite way as its physiological role. Perfusion of Nif alone had no effect on JHCO 3 ‐ and was unable to abolish Neo‐dependent JHCO 3 ‐ inhibition, showing that L‐type Ca 2+ channels are not involved in this effect. These data show that CaSR‐dependent cell Ca 2+ entry via TRP channels inhibit JHCO 3 ‐ . Financial support: FAPESP; CNPq