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Role of Hemodynamic Factors in the Progression of CKD Following AKI
Author(s) -
Polichnowski Aaron,
Griffin Karen,
Picken Maria,
Long Jianrui,
Williamson Geoffrey,
Bidani Anil
Publication year - 2015
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.29.1_supplement.808.20
Subject(s) - medicine , hemodynamics , proteinuria , urology , renal function , glomerulosclerosis , cardiology , ischemia , endocrinology , kidney
The progression of CKD following AKI in rats with renal mass reduction (RMR) may be related to hemodynamic factors; however a rigorous examination of the quantitative relationships between BP and renal injury following AKI in states of RMR has not been performed. In this study, male Sprague‐Dawley rats underwent right uninephrectomy and were instrumented with a BP radiotelemeter. Two weeks later rats were subjected to 40 min ischemia‐reperfusion (IR) or sham IR. BP (every 10 min., 24‐hr/day) and proteinuria (every 4 wks) were assessed over 16 wks following IR (n=22) or sham IR (n=8). Glomerulosclerosis (GS) was assessed in a blinded fashion. Modest increases in systolic BP and robust increases in proteinuria were observed by 12 weeks in IR vs. sham IR rats. Moreover, %GS was greater in IR vs. sham IR rats (10±2% vs. 2±1%) and a relatively low BP threshold (~128 mmHg systolic BP) for the development of GS was evident. At 4 wks post IR (n=12) or sham IR (n=10), BP, RBF and GFR were assessed in additional conscious chronically instrumented (BP radiotelemeter, RBF probe, and FITC‐inulin pumps) rats over several days, following which RBF autoregulatory responses were assessed under anesthesia. RBF was reduced in IR vs. sham IR rats (13±1 vs. 19±1 ml/min), but no significant differences in GFR (2.2±.2 vs. 2.5±.2 ml/min) or autoregulatory index (0.5±.1 vs. 0.4±.1) were noted between groups, respectively. Nevertheless, a significant (p<0.05) inverse correlation (y=‐0.4+1.3, r 2 =0.4) was observed between GFR and autoregulatory index in IR rats. These data strongly support an important role for hemodynamic factors in the progression of CKD following AKI in rats with RMR.