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Antiproliferative Effect of Methanolic Extract of Azadirachta indica on Vascular Smooth Muscle Cells (VSMCs)
Author(s) -
Omobowale Temidayo,
Oyagbemi Ademola,
Adedapo Adeolu,
Yakubu Momoh
Publication year - 2015
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.29.1_supplement.803.4
Subject(s) - vascular smooth muscle , pharmacology , oxidative stress , cell growth , inflammation , antioxidant , chemistry , phytochemical , traditional medicine , medicine , biochemistry , smooth muscle
Azadirachta indica (AI) has been used since antiquity against various diseases characterized by inflammation, proliferation, and oxidative stress. AI is believed to possess anti‐inflammatory, hepatoprotective, antioxidant, and anticarcinogenic properties due to its phytochemical constituents. We investigated the effects of AI on Ang II and LPS‐induced proliferation of VSMCs. Confluent VSMCs were treated with Ang II (10‐6 ‐10‐6 M) or LPS (100 µg/ml) in the presence or absence of AI (25‐100µg/ml). Cellular proliferation was determined using MTT assay and effects of treatments on NO production were evaluated using GRIESS assay. Treatment of confluent VSMC with AI reduced proliferation while Ang II and LPS‐induced VSMC growth. Ang II and LPS enhanced cell proliferations compared to the control. Also, AI treatment mitigated LPS induced production of NO by the VSMC. Ang II and LPS are mediators of various disease conditions ranging from cardiovascular dysfunction to reno‐hepatic diseases characterized by inflammation, cellular proliferation, and free radical generation. Treatment with AI significantly attenuated the effects of Ang II and LPS on VSMC proliferation and NO production suggesting that AI had the potential to combat possible Ang II and LPS mediated vascular dysfunction. Further studies are warranted to identify specific alkaloids and also the phytochemicals involved in the action of AI need to be identified.