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Collagen‐binding Peptide Attenuates Catheter‐Induced Coronary Vasospasm
Author(s) -
Kole Ayeeshik,
Noblet Jill,
Ramchandani Neal,
Tune Johnathan,
Panitch Alyssa,
Sturek Michael
Publication year - 2015
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.29.1_supplement.803.3
Subject(s) - vasospasm , medicine , bradykinin , vasodilation , coronary arteries , coronary vasospasm , lumen (anatomy) , endothelium , cardiology , anesthesia , artery , subarachnoid hemorrhage , receptor , coronary angiography , myocardial infarction
Catheter‐induced coronary vasospasm is a common occurrence in clinical practice and necessites vasodilation via nitroglycerin (NTG). We determined whether a novel peptidoglycan, DS‐SILY 20 , would dilate spastic right coronary arteries (RCA) of Ossabaw miniature swine. DS‐SILY 20 consists of 20 type I collagen‐binding peptides bound to a dermatan sulfate backbone and prevents platelet binding to sub‐endothelial collagen. Pre‐vasospasm lumen diameter of the RCA was measured via angiography. Subsequently, an intravascular ultrasound catheter was introduced to the RCA, triggering spasm of the artery, visualized via angiography. One minute after initial vasospasm, NTG or DS‐SILY (all n =3) was administered via intracoronary bolus over 1 minute. Angiograms were captured at 2 and 10 minutes post‐vasospasm. Arteries exposed to DS‐SILY 20 had significantly greater lumen diameter at 10 minutes post‐vasospasm compared to arteries administered saline ( p <0.05) and similar lumen diameter as NTG‐treated arteries (60±6% saline, 83±3% DS‐SILY 20 , 81±3% NTG). We harvested the distal segments of spastic RCAs and investigated the integrity of the endothelium by pre‐constricting with a thromboxane A 2 agonist followed by increasing doses of the endothelium‐dependent vasodilator bradykinin. We observed a dose‐dependent response to bradykinin ( n =4), comparable to normal control, indicating that the endothelium is uninjured after catheter introduction. DS‐SILY 20 ‐mediated dilation after vasospasm may be through an undiscovered endothelium‐dependent mechanism. Funding: NIH HL062552, HL106792, T32 GM077229