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Enhanced p47 phox NADPH sub‐unit expression impairs conductance and resistance vascular function in obese mice
Author(s) -
AitAissa Karima,
Kassan Modar,
Trebak MOHAMED,
MATROUGUI KHALID,
belmadani souad
Publication year - 2015
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.29.1_supplement.802.3
Subject(s) - nadph oxidase , phenylephrine , nicotinamide adenine dinucleotide phosphate , endocrinology , medicine , nox4 , insulin resistance , enos , metabolic syndrome , oxidase test , chemistry , vascular smooth muscle , p22phox , blood pressure , biology , obesity , biochemistry , oxidative stress , nitric oxide synthase , enzyme , nitric oxide , smooth muscle
Metabolic disease is associated with vascular complication. However, the direct contribution and role of nicotinamide adenine dinucleotide phosphate (NADPH) oxidase subunit p47 phox in obesity‐induced vascular dysfunction is not fully determined. Thus, p47 phox‐/‐ and control mice were fed with high fat diet (HFD) for three months. Systolic blood pressure was normal in all groups. HFD increased body weight similarly in all groups; however, blood glucose was significantly increased in control mice compared to p47 phox‐/‐ mice. Insulin induced vascular reactivity was significantly impaired in control mice when fed with HFD but not affected in p47 phox‐/‐ mice. Vascular contraction in response to phenylephrine was not affected in all groups. Vascular endothelium‐dependent relaxation (EDR) was impaired in control group fed with HFD but protected in p47 phox‐/‐ mice fed with HFD. The improvement of vascular function was associated with the rescue of eNOS and Akt signaling, and reduction in NOX2 and NOX4 mRNA levels and NADPH oxidase activity. The present study determines that HFD impairs vascular function through the augmented expression and activity of NADPH oxidase subunit p47 phox . Therefore, p47 phox could be an important target to improve vascular function in metabolic syndrome.